简介:The most cited neuronal cel culure supplement, B-27 Supplement is an optimized serum-free supplement used to support the low- or high-densit growth and shor- or long-term vabilty ofembryonic, post-nata. and adut hippocampal and other CNS neurons. B-27 Supplement is provided as a 50X liquid and is intended for use with Neurobasal Medium or NeurobasalA Medium for neuronal cel culture without the need for an astrocyte eeder layer. B-27 Supplement is suitable for use in most neuronal cel applications. For other applications, we provide altemative formulations of B 27 Supplement and Neurobasal media. For established protocols using B-27 Supplement, access our Gibco Neurobiology Protocol Handbook View publications using Gibco neurobiology products at our Gibco CellCite .
简介:"Minimum Essential Medium (MEM)a广泛用于乳动物细胞培养以及转染 DHFR 阴性细胞的筛选。MEM 可用于各种县浮和贴整的电乳动物细胞,包括角质形成细胞、原代大星形胶质细胞和人黑色素细胞。我们提供了多种不同的 Gibco@ MEM 改良培养基,适合广泛的细胞培养应用。使用培养基选择工具查找适合的配方。这种 MEM 的改良方式如下: 包含·核糖核昔·脱核糖核·酚红·GutaMAX"
简介:Description: The 61D3 monoclonal antibody reacis with human CD14, a 53-55 kDa GP-inked glycoprotein. CD14 is expressed on monocytes.nterfollicular macrophages and some dendritic cells.Complexes of LPS and LBP (LPS-Binding Protein) bind with high afinity to monocytes throughthe surface CD14. Applications Repoted: The 61D3 antibody has been reported for use in fow cytometic analysis.Applications Tested: This 61D3antibody has been pre-titrated and tested by flow cytometric analysis of normal human peripheral blood cells. This can be used at 5 p. (0.25 ug)Der test.A test is defined as the amount (ug) of antibody that will stain a cel sample in a final volume of 100 pl. Cell number should be determinedempirically but can ranoe from 1045 to 10'8 cells/test. Excitation: 633-647 nm: Emission: 660 nm: Laser. Red laser. Filtration: 02 um post- manufacturing filtered.
简介:MA1-934 detects human, bovine, mouse, and rat calcium sensing receptor. MA1-934 has been successfuly used in Westemn blotmmunohistochemistry, and ELSA procedures. By Wester blot, this antibody detects an 150 and 130 kDa proteins from human HEK-293 cells. Thisantibody has also been used in immunohistochemical on frozen bovine parathyroid gland and rat thyroid gland. The MA1-934 immunogen is a syntheticpeptide corresponding to residues (214) A D D D Y G R P GE KF RE E AE E R D1(235) of human calcium sensing receptor."